会议论文

中文

2016

239-247

第八次全国中西医结合中青年学术论坛

2016-06-03

中国河南郑州

本校为第一机构

目的利用全基因组DNA甲基化芯片,检测蜈蚣提取物（Centipede Scolopendra extraction,CSE）处理后人肝癌细胞全基因组水平DNA甲基化谱情况,并对全基因表达谱芯片的差异基因进行注释系统（gene onto1ogy,G0）分析。方法 MTS法检测CSE对人肝癌细胞HCCLM3增殖的影响,并计算细胞增殖半数抑制率(IC50)。以1/2 IC50浓度的CSE连续

　　Objective: Discuss the different of the genome-wide methylation research of malignant liver cancer cells which treated with the Centipede Scolopendra extraction(CSE) with the whole genome DNA methylation chip and analyse the differences of gene expression profile chip with gene annotation system (gene ontology, GO).Methods: The cellular proliferation was assessed with MTS in liver cancer cells HCCLM3 used with the CSE.Calculate the ICs0 alue concentration.CSE sequential processing with 1/2 ICs0 con-centration of the cells in 3 d, blank comp...