期刊论文

中文

中国现代应用药学

1007-7693

2018

35

5

698-703

10.13748/j.cnki.issn1007-7693.2018.05.017

本校为第一机构

目的探索工业色谱法纯化山银花中绿原酸的最优工艺参数。方法以绿原酸的转移率为评价指标,以山银花水提液过大孔树脂后的20%乙醇洗脱液为原料液,采用单因素及响应面设计试验,对采用工业色谱纯化山银花中绿原酸工艺中的原料液浓缩程度、固定相类型、流动相乙醇浓度、乙醇流速、进样量等主要工艺参数进行优化,确定了最优工业色谱纯化山银花中绿原酸的工艺条件。结果工业色谱纯化山银花中绿原酸的最优工艺条件:以60 ℃,-0.1 MPa为浓缩条件,将原料液浓缩至原体积的0.166 7(1/6),工业色谱UV检测波长为230 nm,以PIPO-02色谱填料为固定相,以18.54%乙醇作为流动相,流速为14.57 mL·min~(-1),进样量为13....

OBJECTIVE To explore the optimal process of the purification by industrial chromatography of chlorogenic acid in Lonicera macranthoides. METHODS The transport rate of chlorogenic acid was used as main assessment index, the eluent of 20% ethanol solution of macroporous resin, which first gained from the water extract solution of Lonicera macranthoides, was used as raw material, single factor and response surface test was adopt to design this test, these main factors, which have effect on the process of the purification by industrial chromatograp...