期刊论文

Association of susceptibility to knee osteoarthritis and related metalloproteinases gene polymorphisms

中文

中华关节外科杂志(电子版)

1674-134X

2020

14

04

454-460

10.3877/cma.j.issn.1674-134X.2020.04.011

国家中医药管理局“平乐郭氏正骨流派传承工作室”建设项目（[2012]228）； 河南省科技厅“国家非物质文化遗产—平乐郭氏正骨术的系统研究与开发”项目（112102310008）； 河南省中医药管理局“河南省中医临床学科领军人才培育计划”项目（HNZYLJ20131009）；

本校为第一机构

目的探讨带有血小板凝血酶敏感蛋白样模体的解整链蛋白金属蛋白酶5(ADAMTS-5)基因单核苷酸多态性(SNP)与膝骨关节炎的关联性。方法连续收集洛阳正骨医院188例膝骨关节炎患者作为病例组,对照组收集排除疾病诊断的100人。应用基因组变异服务器(GVS)网站选择ADAMTS-5基因共计15个SNP位点,采用基质辅助激光解析飞行时间质谱分析技术(MALDI-TOF-MS)进行SNP分型,先行哈迪温伯格(HW)平衡检测,对符合HW平衡的SNP位点等位基因进行卡方检验及单体型分析,对基因型进行logistic回归分析。结果病例组rs2249350位点C等位基因显著多于对照组[比值比(OR) = 1.176,9 5%置信区间(CI) (1.025,1. 351),P = 0.016],A等...

Objective To investigate the relationship between knee osteoarthritis (KOA) susceptibility and single nucleotides polymorphism (SNP) of a disinterring and metalloproteinase with thrombospondin motifs 5 (ADAMTS-5) gene. Methods A total of 188 patients with KOA from Luoyang Orthopedic Hospital were collected as the case group,and 100 patients in the control group were excluded from disease diagnosis. Fifteen SNP of ADAMTS-5 genes were chosen by Genome Variation Server (GVS) online gene database. SNP typing was identified by matrix-assisted laser desorption ionization time-of-flight mass spectrom...