期刊论文

Effect of “treating liver by nourishing spleen”on gut microbiota in rats with liver fibrosis based on Xiaoyao powder and its separated recipe

中文

临床肝胆病杂志

1001-5256

2016

32

4

657-662

10.3969/j.issn.1001-5256.2016.04.009

国家中医临床研究基地业务建设科研专项（JDZX2012062） 湖南省科技厅科技计划（2013SK3105） 湖南省中医药科研基金重点课题（201205）

本校为第一机构

目的 通过逍遥散及其拆方对肝纤维化大鼠肠道菌群结构、门静脉内毒素水平的变化,探讨“肝病实脾法”抗肝纤维化的可能作用机制.方法 将健康Wistar大鼠70只随机分为空白组10只,模型组、实验组和对照组各20只,采用牛血清白蛋白尾静脉注射8周建立免疫性肝纤维化大鼠模型.实验组予逍遥散中药颗粒剂进行灌胃治疗,对照组予逍遥散去健脾类中药颗粒剂灌胃.观察各组血清转氨酶、肝组织病理学、门静脉内毒素及肠道菌群指纹图谱分析(ERIC-PCR).满足方差齐性条件的计量资料多组间比较采用方差分析,进一步2组间比较采用LSD-t检验,方差不齐时用Tamhane's法.相关性检验采用Pearson相关分析.结果 与空白组比较,模...

Objective To investigate the possible mechanism of＂treating liver by nourishing spleen＂in the treatment of liver fibrosis with reference to the effect of Xiaoyao powder and its separated recipe on gut microbiota and the level of portal endotoxins. Methods A total of70 healthy Wistar rats were randomly divided into blank group（ 10 rats）,model group（ 20 rats）,experimental group（ 20 rats）,and control group（ 20 rats）,and tail vein injection of bovine serum albumin was performed for 8 weeks to establish a rat model of immune liver fibrosis...