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Systematic biological and proteomics strategies to explore the regulation mechanism of Shoutai Wan on recurrent spontaneous Abortion's biological network

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成果类型:
期刊论文
作者:
Zeng, Liuting;Yang, Kailin;Liu, Liting;Zhang, Tianqing;Liu, Huiping*;...
通讯作者:
Zeng, LT;Yang, KL;Liu, Huiping;Lei, L
作者机构:
[Zeng, Liuting; Zeng, LT] Chinese Acad Med Sci & Peking Union Med Coll, Peking Union Med Coll Hosp, Dept Rheumatol & Clin Immunol, Beijing, Peoples R China.
[Zeng, Liuting] Minist Educ, Key Lab Rheumatol & Clin Immunol, Beijing, Peoples R China.
[Yang, KL; Yang, Kailin] Capital Med Univ, Beijing, Peoples R China.
[Yang, Kailin] Capital Med Univ, Beijing Anzhen Hosp, Beijing, Peoples R China.
[Liu, Liting] Huazhong Univ Sci & Technol, Wuhan, Hubei, Peoples R China.
通讯机构:
[Zeng, LT ; Yang, KL ] C
[Lei, L ; Liu, HP] H
Chinese Acad Med Sci & Peking Union Med Coll, Peking Union Med Coll Hosp, Dept Rheumatol & Clin Immunol, Beijing, Peoples R China.
Capital Med Univ, Beijing, Peoples R China.
Hunan Univ Chinese Med, Changsha, Hunan, Peoples R China.
语种:
英文
关键词:
actin depolymerizing factor;agents acting on the genital system;alpha crystallin;alpha enolase;asperosaponin vi;calreticulin;Chinese medicinal formula;chlorogenic acid;coumaric acid;heat shock protein 27;heat shock protein beta 1;hyperin;kaempferol;lipocortin 1;lipocortin 2;lipocortin 5;peroxiredoxin 6;phosphoinositide dependent protein kinase 1;proteome;quercetin;quercitrin;saponin derivative;serotonin 2B receptor;serum albumin;shoutai wan;transferrin;transthyretin;tropomyosin 4;ubiquitin protein ligase E3;unclassified drug;vimentin;herbaceous agent;animal cell;animal experiment;animal model;animal tissue;Article;bioinformatics;biological activity;CBA mouse;controlled study;DBA 2 mouse;drug determination;drug dose comparison;drug mechanism;drug megadose;drug targeting;embryo;embryo death;female;image analysis;immunohistochemistry;isoelectric point;low drug dose;male;matrix assisted laser desorption ionization time of flight mass spectrometry;mouse;nonhuman;peptide mass fingerprinting;proteomics;quality control;recurrent abortion;retention time;spontaneous abortion;synaptic transmission;two dimensional electrophoresis;two dimensional gel electrophoresis;Western blotting;animal;Bagg albino mouse;DBA mouse;drug effect;gene regulatory network;genetics;isolation and purification;matrix-assisted laser desorption-ionization mass spectrometry;physiology;pregnancy;procedures;proteomics;recurrent abortion;Abortion, Habitual;Animals;Drugs, Chinese Herbal;Female;Gene Regulatory Networks;Male;Mice;Mice, Inbred BALB C;Mice, Inbred CBA;Mice, Inbred DBA;Pregnancy;Proteomics;Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
期刊:
Journal of Ethnopharmacology
ISSN:
0378-8741
年:
2020
卷:
263
页码:
113156
基金类别:
statement This research did not receive any specific grant from funding agencies in the public, commercial, or not-for-profit sectors. Acknowledgments This research did not receive any specific grant from funding agencies in the public, commercial, or not-for-profit sectors.
机构署名:
本校为通讯机构
摘要:
Ethnopharmacological relevance: Shoutai Wan (STW) is a classic herbal formula for the treatment of recurrent spontaneous abortion (RSA), and clinical studies have shown the effectiveness of STW on RSA. However, the molecular mechanism of STW treatment of RSA is still unclear. Methods: (1) Animal experiments: The normal pregnancy model was established with CBA/J*BALB/C, and the RSA model was established by CBA/J*DBA/2. The RSA model CBA/J*DBA/2 pregnant mice were randomly divided into four groups (RSA model group, STW low, medium and high dose g...

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