期刊论文

Shen, GL

中文

分析化学

0253-3820

2003

31

8

897-900

10.3321/j.issn:0253-3820.2003.08.001

国家自然科学基金 （No .2 0 0 75 0 0 6 ,2 0 2 0 5 0 0 4）； 高等学校博士点基金 （No .2 0 0 10 5 32 0 0 8）资助项目；

本校为其他机构

提出了一种基于巯基自组装的新型蛋白质固定化方法.应用偶联试剂碳二亚胺(EDC)和N-羟基琥珀酰亚胺(NHS)使聚丙烯胺盐酸盐(PAH)与3-巯基丙酸(MPA)偶合,在石英晶振表面自组装,形成一带多氨基的巯基自组装膜.应用该方法成功地固定了羊抗人IgM抗体,并用于人血清免疫球蛋白M(IgM)的测定.详细考察了自组装条件、抗体包被和免疫反应的主要实验条件以及传感器的响应性能.与MPA法比较,应用该方法可在传感器表面固定更多的抗体分子,传感器的响应灵敏度亦更好.在优化的实验条件下,反应的线性范围0.66～26.4 mg/L;回归方程Y=92.44+12.40X;相关系数r=0.9920.

A novel protein immobilization approach for piezoelectric immunosensors has been proposed, which is based on the self-assembled membrane (SAM) of mercapto-poly (allylamine hydrochloride) (PAH) formed by coupling mercaptopropionic acid (MPA) to PAH via 1-ethyl-3-(3-dimethylamino-propl) carbodiimide (EDC) and N-hydroxysuccinimide (NHS). After self-assembling a mercapto-PAH SAM on the gold electrode of the quartz crystal, the goat-anti-IgM antibody is immobilized via cross-linking with glutaraldehyde. Comparing with the MPA SAM method, this new approach shows greater frequency response and higher...