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Sensitive Detection of RNase A Activity and Collaborative Drug Screening Based on rGO and Fluorescence Probe

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成果类型:
期刊论文
作者:
Tong, Chunyi;Zhao, Chuan;Liu, Bin*;Li, Bin;Ai, Zhaoyang;...
通讯作者:
Liu, Bin;Wang, Wei
作者机构:
[Liu, Bin; Fan, Jialong; Zhao, Chuan; Tong, Chunyi] Hunan Univ, Hunan Prov Key Lab Plant Funct Genom & Dev Regula, State Key Lab Chem Biosensing & Chemometr, Coll Biol, Changsha 410082, Hunan, Peoples R China.
[Li, Bin; Wang, Wei] Hunan Univ Chinese Med, Sch Pharm, TCM & Ethnomed Innovat & Dev Lab, Changsha 410208, Hunan, Peoples R China.
[Ai, Zhaoyang] Hunan Univ, Inst Cognit & Biophys Linguist, Coll FLs, Changsha 410082, Hunan, Peoples R China.
通讯机构:
[Liu, Bin; Wang, Wei] H
Hunan Univ, Hunan Prov Key Lab Plant Funct Genom & Dev Regula, State Key Lab Chem Biosensing & Chemometr, Coll Biol, Changsha 410082, Hunan, Peoples R China.
Hunan Univ Chinese Med, Sch Pharm, TCM & Ethnomed Innovat & Dev Lab, Changsha 410208, Hunan, Peoples R China.
语种:
英文
期刊:
Analytical Chemistry
ISSN:
0003-2700
年:
2018
卷:
90
期:
4
页码:
2655-2661
基金类别:
Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [81374062, 81673579, 31672457]; Hunan Province Universities Collaborative Innovation Center of Protection and Utilization of Hu-xiang Chinese Medicine Resources; National Standardization Project of Traditional Chinese Medicine [ZYBZH-Y-HUN-23]
机构署名:
本校为通讯机构
院系归属:
药学院
摘要:
In addition to being an important object in theoretical and experimental studies in enzymology, RNase A also plays an important role in the development of many kinds of diseases by regulating various physiological or pathological processes, including cell growth, proliferation, differentiation, and invasion. Thus, it can be used as a useful biomarker for disease theranostics. Here, a simple, sensitive, and low-cost assay for RNase A was constructed by combining a fluorogenic substrate with reduced graphene oxide (rGO). The method with detection limit of 0.05 ng/mL was first applied for RNase A...

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