期刊论文

Xiong Hui

中文

数字中医药(英文)

2096-479X

2019

2

1

29-40

10.1016/j.dcmed.2019.05.004

We thank for the funding support from the National Natural Science Foundation of China (No. 81574005 and No. 81874478 ).

本校为第一且通讯机构

目的尽管骨髓间充质干细胞的应用在骨科领域展现出巨大的潜力,但因移植后面临着严峻的氧化应激的环境,使得其应用受到限制。本实验将研究紫草酸对过氧化氢诱导的骨髓间充质干细胞早期凋亡的抗凋亡和抗氧化作用的研究。方法本次研究使用过氧化氢诱导的氧化应激的细胞模型,利用相关试剂盒检测间充质干细胞内活性氧和超氧化物歧化酶水平,蛋白免疫印迹检测胞内p-AKT、Bax、Bcl-2的蛋白表达情况。结果紫草酸能显著抑制过氧化氢诱导的细胞核固缩,降低胞内活性氧的水平和增强超氧化物歧化酶的活性;而且,它抑制过氧化氢诱导的骨髓间充质干细胞凋亡与上调Bcl-2、p-Akt蛋白表达,下调Bax蛋白表达有关,其抑制...

Objective: Despite the potential therapeutic approaches of bone marrow-derived mesenchymal stem cells (BMSCs) in orthopaedic, their applications are hampered by harsh oxidative stress conditions after transplantation. In this study, the anti- apoptotic and anti-oxidative properties of lithospermic acid (LSA) on BMSCs exposed to hydrogen peroxide (H2O2) were investigated. Methods: In the present study, we used H2O2 to induce oxidative injury on BMSCs. Reactive oxygen species (ROS) staining and superoxide dismutase (SOD) assay were performed. The...