期刊论文

GDF11 affects M1 polarization mediated inflammatory response in macrophages by regulating the NF-κB pathway

中文

中国医科大学学报

0258-4646

2024

53

4

342-348,354

10.12007/j.issn.0258-4646.2024.04.009

2022JJ80065:湖南省自然科学基金科药联合项目 202110000505:湖南省卫生健康委员会一般指导项目 BSJJ202103:湖南省人民医院仁术国自培育项目

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目的探讨生长转化因子11(GDF11)对巨噬细胞M1极化介导的炎症反应的影响及其作用机制。方法体外培养RAW264.7巨噬细胞,100 ng/mL LPS+30 ng/mL IFN-γ诱导巨噬细胞M1极化,并将细胞分成对...展开更多 目的探讨生长转化因子11(GDF11)对巨噬细胞M1极化介导的炎症反应的影响及其作用机制。方法体外培养RAW264.7巨噬细胞,100 ng/mL LPS+30 ng/mL IFN-γ诱导巨噬细胞M1极化,并将细胞分成对照组、LPS+IFN组、GDF11组及LPS+IFN+GDF11组。流式细胞术检测巨噬细胞的极化,实时PCR检测IL-6、TNF-α、NF-κB及GDF11 mRNA的表达水平,Western blotting检测细胞GDF11、NF-κB及P-NF-κB蛋白表达水平,免疫荧光检测...

Objective To investigate the effect of GDF11 on the macrophage M1 polarization mediated inflammatory response and its mechanism.Methods RAW264.7 macrophages were cultured in vitro,and M1 polarization was induced by 100 ng/mL LPS+30 ng/mL IFN-γ.The cells were divided int...MORE Objective To investigate the effect of GDF11 on the macrophage M1 polarization mediated inflammatory response and its mechanism.Methods RAW264.7 macrophages were cultured in vitro,and M1 polarization was induced by 100 ng/mL LPS+30 ng/mL IFN-γ.The cells were divided int...