期刊论文

中文

中国生物化学与分子生物学报

1007-7626

2012

28

8

722-727

10.13865/j.cnki.cjbmb.2012.08.003

国家自然科学基金（No.31071150）资助项目~~；

本校为其他机构

TRAF2是机体免疫与炎症反应中具有重要作用的蛋白,有E3连接酶活性;GFP是一种受到激发光照射后可产生绿色荧光的蛋白,常用于蛋白质的细胞共定位研究,并显示与GFP融合表达靶蛋白在细胞中的位置.我们实验发现共转染表达质粒pCMV-myc-TRAF2与pEGFP-C3,可引起转染细胞绿色荧光减弱.Western印迹实验证明TRAF2可以降解GFP,并且这种降解是通过蛋白酶体途径进行的.为进一步确定这种降解的特异性,在HEK293细胞中共转pCMV-myc-TRAF2与pEGFP-C3-LNX后,发现随着TRAF2表达量增加,融合蛋白GFP-LNX减少;而共转质粒pCMV-myc-TRAF2与pCMV-myc-LNX后,未发现LNX蛋白表达减少,表明TRAF2对GFP的降解具有相对特异性.GFP...

TRAF2 with E3 ligase activity is an important protein in immunity and inflammation.GFP was often expressed in fusion protein for co-localizing the distribution of target proteins.We found reduced green fluorescence in pCMV-myc-TRAF2 co-transfected cells.The increased degradation of GFP by TRAF2 was detected by Western blotting,and further experiment showed that the degradation was through the proteasome pathway.By co-transfecting with pCMV-myc-TRAF2 plus pEGFP-C3-LNX,we showed a TRAF2-related reduction of GFP-LNX expression,but not LNX.These results suggested that the degradation of GFP promot...