期刊论文

Ning, Yi(121407735@qq.com)

中文

中国环境科学

1000-6923

2018

38

2

737-744

10.19674/j.cnki.issn1000-6923.2018.0087

湖南省自然科学青年科学基金资助项目（2016JJ3098）； 湖南省教育厅重点项目（16A027）； 湖南省中医管理局重点项目基金资助项目（201711）；

本校为通讯机构

设计新型的DNA探针,其主要功能区域包含适配体区域和分子信标互补区域;当无靶标存在时,羧基荧光素(FAM)的荧光被黑洞猝灭基团(BHQ)猝灭,加入靶标后,Ag~+与适配体区域特异性结合,使得分子信标与其互补区域发生结合,加入EXO III后,分子信标被酶解,从而释放Ag~+/DNA探针复合物用于下一轮反应,同时产生大量的FAM,因此,可以通过荧光的增强来定量检测靶标的含量.评价了该方法的灵敏度和选择性,探究它在实际样品中的表现能力.结果表明,适配体区域与分子信标间隔2个碱基的DNA探针与分子信标结合效果最好,检测的信噪比最大.该方法对Ag~+的检测最低下限(LOD)为0.1nmol/L,在0.5~200nmol/L范围内,荧光值与靶...

To establish a lower cost, rapid, higher sensitive and specifical detection method for Ag+, we designed a novel probe containing the aptamer region and the complementary region of molecular beacon (MBs). In the absence of target, the fluorescence of FAM was quenched by BHQ. Upon adding the target, it would specifically bind to the aptamer, leading to the complementary region being recognized by MBs. MBs were cleaved when the EXO III was introduced, which can release Ag+/DNA complex for the next round of reaction and free FAM for signal amplification,simultaneously. Hence, the amount of targets...