期刊论文

Effects of telmisartan on left ventricular hypertrophy related protein profiles in spontaneously hypertensive rats

中文

中华高血压杂志

1673-7245

2020

28

8

750-756

10.16439/j.cnki.1673-7245.2020.08.016

81774207:国家自然科学基金 81973753:国家自然科学基金 2018JJ2291:湖南省自然科学基金

本校为第一机构

目的 研究替米沙坦对自发性高血压大鼠(SHR)左心室心肌组织蛋白质谱的影响.方法 将16只SHR随机分为对照组、替米沙坦组,分别给予无菌注射用水(10 mL/kg)和替米沙坦(4.33mg/kg)进行灌胃,另外选取8只Wistar-Kyoto(WKY)大鼠给予无菌注射用水(10 mL/kg)进行灌胃(WKY组),连续给药12周.12周后处死大鼠,取心脏样本制成蛋白质剂.随后将蛋白质剂分离部分进行同位素标记相对和绝对定量(iTRAQ)、强阳离子交换柱液相分离并用串联质谱仪进行质谱鉴定.最后通过差异蛋白通路富集分析选出与通路相关的蛋白质及其参与的生化代谢途径和信号转导途径.结果 与通路相关的总共23个差异蛋白,与替米沙坦组比较,SHR组上调...

Objective To investigate the effects of telmisartan on the protein profiles of left ventricular myocardial tissue in spontaneously hypertensive rats(SHR). Methods Sixteen SHR were randomly divided into the control group and the telmisartan group. They were treated with sterile water for injection(10mL/kg)or telmisartan (4.33mg/kg)by intragastric administration. Wistar-Kyoto(WKY)rats treated with sterile water for injection (10mL/kg)served as control. The treatment period was 12weeks. After 12weeks,those rats were sacrificed, and their heart sam...