期刊论文

Liao, Duan-fang

英文

中国药理学报

1671-4083

2011

32

1

45-51

10.1038/aps.2010.183

American Heart AssociationAmerican Heart Association [0565196B]; NIHUnited States Department of Health & Human ServicesNational Institutes of Health (NIH) - USA [DK074995, R01HL102042]; National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [30971170, 30770868, 30971267, 30600249]; Outstanding Youth Foundation of Education Department in Hunan Province [09B089]; NATIONAL HEART, LUNG, AND BLOOD INSTITUTEUnited States Department of Health & Human ServicesNational Institutes of Health (NIH) - USANIH National Heart Lung & Blood Institute (NHLBI) [R01HL102042, R01HL102042, R01HL102042, R01HL102042, R01HL102042, R01HL102042, R01HL102042] Funding Source: NIH RePORTER; NATIONAL INSTITUTE OF DIABETES AND DIGESTIVE AND KIDNEY DISEASESUnited States Department of Health & Human ServicesNational Institutes of Health (NIH) - USANIH National Institute of Diabetes & Digestive & Kidney Diseases (NIDDK) [R21DK074995, R21DK074995] Funding Source: NIH RePORTER

本校为其他机构

Aim:To evaluate the effects of angiopoietin-1 (Ang-1) on myocardial endothelial cell function under high glucose (HG) condition.Methods:Mouse heart myocardial endothelial cells (MHMECs) were cultured and incubated under HG (25 mmol/L) or normal glucose (NG, 5 mmol/L) conditions for 72 h. MTT was used to determine cellular viability, and TUNEL assay and caspase-3 enzyme linked immunosorbent assays were used to assay endothelial apoptosis induced by serum starvation. Immunoprecipitation and Western blot analysis were used to analyze protein phosp...