期刊论文

Curculigo orchioides extract-induced activation of RAW264. 7 cell

中文

中药药理与临床

1001-859X

2017

33

5

87-91

10.13412/j.cnki.zyyl.2017.05.023

国家自然基金课题（NO：81460597） 贵州省科技厅与贵阳中医学院联合基金项目（黔科合LH字[2016]7109号） 湖南省高校科技创新团队《感染性疾病中医药防治研究》资助项目（NO：15）

本校为第一机构

目的:研究仙茅提取物对小鼠巨噬细胞株RAW264. 7的诱导活化作用。方法:应用水提醇沉方式制备仙茅提取物,其中多糖含量为50%。将不同浓度的仙茅提取物(31. 25 ~ 500μg /ml)作用于RAW264. 7细胞36h后,采用流式细胞术检测膜表面模式识别受体TLR2 /4和MR的表达;实时荧光定量PCR(RT-PCR)检测细胞iNOS和TNF-α的mRNA表达水平;收集培养上清液,用ELISA法测定中TNF-α含量,Griess法检测NO的含量。结果:仙茅提取物的剂量在500μg /ml以下时,对细胞增殖影响不明显;与空白对照组相比,浓度高于62. 5μg /ml的仙茅提取物刺激RAW264. 7细胞可使TNF-a、iNOS mRNA表达量显著升高,促进细胞分泌活性因子TNF-α和NO...

Objective: To discuss how Curculigo orchioides extract plays roles in activation of RAW264. 7 through secretion and mRNA transcription of TNF-α and NO,expression of PRRs. Methods: We prepared the extract from Curculigo orchioides by water and alcohol. RAW264. 7 cells were incubated with various concentrations of Curculigo orchioides extract (31. 25 ~ 500mg /L) ,TNF-α secretion were measured by ELISA kits; NO secretion was measured by Griess ; TNF-a and iNOS mRNA transcription were evaluated by RT-PCR. As well,the expression of PRRs was determ...