Hairy root induction and benzylisoquinoline alkaloid production in Macleaya cordata

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成果类型：

期刊论文

作者：

Huang, Peng;Xia, Liqiong;Liu, Wei;Jiang, Ruolan;Liu, Xiubin;...

通讯作者：

Zeng, JG

作者机构：

[Liu, Xiubin; Liu, Wei; Jiang, Ruolan; Tang, Qi; Xu, Min; Zeng, Jianguo; Huang, Peng; Yu, Linlan] Hunan Agr Univ, Hunan Key Lab Tradit Chinese Vet Med, Changsha 410128, Hunan, Peoples R China.

[Liu, Xiubin; Liu, Wei; Jiang, Ruolan; Tang, Qi; Xu, Min; Zeng, Jianguo; Huang, Peng; Yu, Linlan] Hunan Agr Univ, Coll Hort & Landscape, Changsha 410128, Hunan, Peoples R China.

[Xia, Liqiong] Hunan Univ Chinese Med, Sch Pharm, Changsha 410208, Hunan, Peoples R China.

[Liu, Xiubin; Liu, Wei] Hunan Agr Univ, Ctr Analyt Serv, Changsha 410208, Hunan, Peoples R China.

[Tang, Zhaoshan] Micolta Bioresource Inc, Changsha 410016, Hunan, Peoples R China.

通讯机构：

[Zeng, JG ] H

Hunan Agr Univ, Hunan Key Lab Tradit Chinese Vet Med, Changsha 410128, Hunan, Peoples R China.

Hunan Agr Univ, Coll Hort & Landscape, Changsha 410128, Hunan, Peoples R China.

Hunan Agr Univ, Natl & Local Union Engn Res Ctr, Vet Herbal Med Resource & Initiat, Changsha 410128, Hunan, Peoples R China.

语种：

英文

期刊：

Scientific Reports

ISSN：

2045-2322

年：

2018

卷：

期：

页码：

11986

DOI：

10.1038/s41598-018-30560-0

基金类别：

National Key R&D Program of China [2017YFD0501500]; talent introduction Science Foundation of Hunan Agricultural University [13YJ09]; Natural Science Foundation of Hunan Province [2016JJ4040]; National key laboratory cultivation base construction project [15KFXM15]; innovation guidance program project of Hunan provincial science and Technology Department [2016SK3002]

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本校为其他机构

院系归属：

药学院

摘要：

Sanguinarine is currently widely used to replace antibiotic growth promoters in animal feeding and has demonstrated useful anticancer activity. Currently, the main source of sanguinarine is from an important medicinal plant, Macleaya cordata. To obtain a new source of sanguinarine production, we established hairy root cultures of M. cordata by co-cultivating leaf and stem explants with Agrobacterium rhizogenes. Except the co-cultivation medium, all growth media contained 200 mg/L timentin to eliminate A. rhizogenes. Through comparing the metabolic profiles and gene expression of hairy roots an...

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Hairy root induction and benzylisoquinoline alkaloid production in Macleaya cordata

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