期刊论文

Effects of Deoxynivalenol on Apoptosis of IPEC-J2 Cells

中文

动物营养学报

1006-267X

2018

30

3

1027-1034

10.3969/j.issn.1006-267x.2018.03.027

国家自然科学基金青年基金（31402088）；

本校为第一机构

本研究以IPEC-J2细胞为材料,研究不同浓度和时间处理下呕吐毒素(DON)对猪空肠上皮细胞凋亡的影响。通过CCK-8试剂盒检测细胞增殖毒性,筛选出合适的DON作用时间(6、12、24 h) 。通过低浓度(200 ng / mL)和高浓度(2 000 ng / mL) DON分别作用IPEC-J2细胞6、12、24 h,检测DON对细胞有丝分裂周期和细胞早期凋亡的影响。结果表明:1) DON对IPEC-J2细胞生长有明显抑制作用,200和2 000 ng / mL DON浓度下,48和72 h的细胞存活率显著低于24 h(P< 0.05);2 000 ng / mL DON浓度下,72 h的细胞存活率显著低于48 h(P < 0.05) 。2)不同DON浓度对细胞周期影响不同,低浓度DON主要作用于细胞有丝分裂S期,干扰DNA的复...

This experiment was conducted to determine the effect of deoxynivalenol (DON) on the apoptosis of IPEC-J2 cells after different concentrations and time treatment. An appropriate exposure time (6, 12, and 24 hour) used CCK-8 assay to test for cell proliferation. The effects of DON on cell mitosis cycle and early cell apoptosis were examined by treated the IPEC-J2 cells with low concentration (200 ng / mL) and high concentrations (2 000 ng / mL) DON for 6, 12, 24 hours. The results showed as follows: 1) DON significantly inhibited the growth of IPEC-J2 cells. At 200 and 2 000 ng / mL DON concent...