期刊论文

Ou, M.E.

中文

中国中西医结合杂志

1003-5370

2009

29

5

430-434

10.3321/j.issn:1003-5370.2009.05.012

国家自然科学基金资助项目（No.30572301） 教育部科学研究重点项目（No.204100） 湖南省教育厅重点资助项目（No.03A033）

本校为第一机构

目的 研究补阳还五汤有效组分生物碱、苷对凝血酶刺激的血管内皮细胞株黏附分子表达的影响及其信号转导机制.方法 (1)体外培养脐静脉内皮细胞株ECV-304细胞,以凝血酶(Thr)刺激,加入原方、生物碱、苷后培养,免疫细胞化学法检测黏附分子PECAM-1(CD_(31))、ICAM-1(CD_(54))、E-selectin和PKCα的表达;(2)培养的ECV-304细胞,分别给予Thr、Thr+H7[1-(5-异喹啉磺酰基)-2-甲基哌嗪]、PMA(佛波脂)、PMA+H7、Thr+CATG(组织蛋白酶-G)、Thr+原方、Thr+生物碱、Thr+苷,免疫细胞化学法检测细胞蛋白激酶Cα(protein kinase C α,PKCα)、磷酸化p38丝裂原活化蛋白激酶(phospho-p38 mitogen-activated protein k...

OBJECTIVE: To explore the effect and possible cell signal transduction mechanism of active components (alkaloids and glucosides) of Buyang Huanwu Decoction (BHD) on the vascular adhesion molecule (VAM) expression induced by thrombin (Thr). METHODS: (1) Endothelial cells (ECV-304) were cultured in vitro and stimulated by Thr, then cultured in media containing BHD whole recipe (BHDw), its alkaloids (BHDa) or glycosides (BHDg), respectively. The expressions of E-selectin, CD31, CD54, and protein Kinase Calpha PKCa) were detected by immunocytochemical method. (2) Cultured ECV-304 cells were treate...