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Screening of Tnfaip1-Interacting Proteins in Zebrafish Embryonic cDNA Libraries Using a Yeast Two-Hybrid System

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成果类型:
期刊论文
作者:
Huang, Shulan;Zhang, Hongning;Chen, Wen;Wang, Jiawei;Wu, Zhen;...
通讯作者:
Hu, X;Xiang, SL
作者机构:
[Zhang, Hongning; Wang, Jiawei; Zhang, Jian; Chen, Wen; He, Meiqi; Hu, Xiang; Wu, Zhen; Hu, X; Huang, Shulan] Hunan Normal Univ, Coll Life Sci, State Key Lab Dev Biol Freshwater Fish, Changsha 410081, Peoples R China.
[Xiang, Shuanglin] Hunan Normal Univ, Coll Life Sci, Engn Res Ctr Antibodies Expt Anim Hunan Prov, Changsha 410081, Peoples R China.
通讯机构:
[Xiang, SL ; Hu, X ] H
Hunan Normal Univ, Coll Life Sci, State Key Lab Dev Biol Freshwater Fish, Changsha 410081, Peoples R China.
Hunan Normal Univ, Coll Life Sci, Engn Res Ctr Antibodies Expt Anim Hunan Prov, Changsha 410081, Peoples R China.
语种:
英文
关键词:
Tnfaip1;zebrafish embryos;cDNA library;yeast two-hybrid system;interacting protein
期刊:
Current Issues in Molecular Biology
ISSN:
1467-3045
年:
2023
卷:
45
期:
10
页码:
8215-8226
基金类别:
Conceptualization, S.H., X.H. and S.X.; methodology, S.H. and X.H.; software, W.C. and H.Z.; validation, S.H., Z.W. and M.H.; formal analysis, S.H. and J.W.; investigation, S.H. and X.H.; resources, S.H., J.Z., X.H. and S.X.; data curation, S.H.; writing—original draft preparation, S.H.; writing—review and editing, S.H., H.Z., W.C., J.W., Z.W., M.H., J.Z., S.X. and X.H; visualization, S.H.; supervision, X.H. and S.X.; project administration, X.H. and S.X.; funding acquisition, X.H. and S.X. All authors have read and agreed to the published version of the manuscript. This research was funded by the National Natural Science Foundation of China (81972642), the Hunan Provincial Natural Science Foundation of China (2021JJ30444), and the Changsha City Natural Science Foundation of China (kq2007013). The APC was funded by the National Natural Science Foundation of China (81972642).
机构署名:
本校为第一机构
院系归属:
医学院
摘要:
TNFAIP1 regulates cellular biological functions, including DNA replication, DNA repair, and cell cycle, by binding to target proteins. Identification of Tnfaip1-interacting proteins contributes to the understanding of the molecular regulatory mechanisms of their biological functions. In this study, 48 hpf, 72 hpf, and 96 hpf wild-type zebrafish embryo mRNAs were used to construct yeast cDNA library. The library titer was 1.12 × 107 CFU/mL, the recombination rate was 100%, and the average length of the inserted fragments was greater than 1000 b...

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