期刊论文

Tian, X.-F.

中文

中草药

0253-2670

2017

48

5

930-934

10.7501/j.issn.0253-2670.2017.05.015

国家自然科学基金资助项目（81473617） 湖南省***卫生人才“225工程”项目资助（湘卫人发2013-13号）

本校为第一且通讯机构

目的观察蜈蚣提取物作用于肝癌HepG2细胞后对信号传导与转录激活因子3(STAT3)信号通路与磷酸化相关蛋白的表达以及细胞转移侵袭能力的影响,探讨蜈蚣提取物介导STAT3信号通路抗肝癌侵袭转移的调控作用机制。方法梯度质量浓度(300、600、1 200、2 400 μg/mL)的蜈蚣提取物处理人肝癌HepG2细胞,采用CCK8法检测细胞增殖抑制率,计算半数抑制浓度(IC_(50))。将人肝癌细胞分为对照组,蜈蚣提取物250、500 μg/mL组,5-氟尿嘧啶(5-FU)对照组,蜈蚣提取物处理人肝癌细胞48 h时,Transwell细胞侵袭实验检测HepG2细胞的侵袭能力变化,Western blotting方法检测STAT3、 p-STAT3、血管内皮生长因子(VEGF)、基质金属...

Objective: To observe the effect of Scolopendra subspinipes extracts (SSE) on signal transducer and activator of transcription 3 (STAT3) signaling pathway and phosphorylation of its protein expression as well as the regulation mechanisms of HepG2 cells proliferation, invasion, and metastasis after SSE exposure. Methods: HepG2 cells were processed with SSE of gradient concentration (300, 600, 1 200, and 2 400 μg/mL). The inhibitory effect of SSE on HepG2 cell proliferation was evaluated by CCK8 method. Subsequent experimental concentration was ...