期刊论文

中文

中国康复理论与实践

1006-9771

2017

23

6

628-633

10.3969/j.issn.1006-9771.2017.06.002

国家自然科学基金面上项目

本校为第一机构

目的观察电针对完全性脊髓损伤后神经源性膀胱大鼠膀胱功能及脊髓组织中Caspase-9、细胞色素C (Cyt-C)及凋亡蛋白酶激活因子-1 (Apaf-1)表达的影响。方法雌性Sprague-Dawley大鼠60只,随机抽取36只,采用改良T10脊髓横断法制作完全性脊髓损伤后神经源性膀胱模型,取24只成功模型分为模型组和电针组各12只,其余未造模大鼠24只随机分为假手术组和空白组各12只。于造模后第19天取次髎、中极、三阴交、大椎行电针,连续7 d后行尿流动力学检测,TUNEL法测定细胞凋亡率, Western blotting测定脊髓组织中Caspase-9、Cyt-C和Apaf-1蛋白的表达情况。结果与空白组和假手术组比较,模型组和电针组膀胱最大容量及膀...

Objective To observe the effect of electroacupuncture on Caspase-9, cytochrome C (Cyt-C) and apoptotic protease activat-ing factor 1 (Apaf-1) protein in rats with neurogenic bladder after complete spinal cord injury. Methods A total of 60 female Sprague-Daw-ley rats were involved, in which 36 rats were randomly selected to establish the neurogenic bladder model with T10 spinal cord transected. 24 successful models selected were randomly divided into model group (n=12) and electroacupuncture group (n=12). The other 24 rats were al-so randomly divided into sham group (n=12) and blank group (n=12...