期刊论文

中文

中药新药与临床药理

1003-9783

2013

24

1

18-21

10.3969/j.issn.1003-9783.2013.01.005

国家自然科学基金资助项目（81072939） 湖南省科技计划项目（2011SK3103） 湖南省自然科学基金资助项目（12JJ5046）.

本校为第一机构

目的探讨补阳还五汤对局灶性脑缺血大鼠脑组织TLR4表达的影响。方法72只大鼠随机分为假手术组、模型组和补阳还五汤组,每组按给药后7、14、21 d 3个时间点再各分3组,用免疫组化、Western blot、RT-PCR法检测各组不同时间点TLR4的蛋白和基因表达。结果模型组和补阳还五汤组各时间点TLR4蛋白和mRNA表达均高于假手术组(P

Objective To observe the effect of Buyang Huanwu Decoction on TLR4 expression in brain tissue of rats with focal cerebral ischemia. Methods Seventy-two SD rats were randomly divided into sham operation group, model group, and Buyang Huanwu Decoction group. Each group was divided into three subgroups according to the time after administration for 7d, 14d and 21d respectively. TLR4 protein and mRNA levels in each group were examined by using immunohistochemistry, Western blotting and RT-PCR respectively. Results Expression of TLR4 protein and mRN...