期刊论文

Luo, H.Y.

中文

细胞与分子免疫学杂志

1007-8738

2011

27

11

1176-1179

10.13423/j.cnki.cjcmi.006197

国家自然科学基金资助项目（31071150）；

本校为其他机构

目的:对TRAF2的新剪切体进行鉴定与表达分析。方法:以人脑库为模板,利用PCR扩增,电泳确定新剪切体的存在。再提取不同细胞与组织的总RNA,逆转录得到cD-NA后作为模板对两种不同剪切体的表达进行比较分析。结果:利用P1与P2引物对从人脑库扩增出1条约1 500 bp的条带,而利用P3与P4引物对则得到2条明显电泳带,通过测序表明其中1条包含TRAF2的第6外显子,另1条缺失了TRAF2的第6外显子。通过对新剪切体TRAF2Δ6与全长剪切体TRAF2的表达量比较表明在T47D中全长剪切体表达占优势;而在Hep3B、GC-1与MCF7中TRATΔ6剪切体表达占优势;在HepG2、HBL100、A549与HeLa细胞中未发现两种剪切体的明显表达;在PANCI与S...

AIM: Identification of a novel splice variant of tumor necrosis factor receptor associated factor 2 and its expression analysis. METHODS: By PCR analysis, we identified the existence of a new splice variant of TRAF2 using human brain cDNA library as template. RNA isolated from various cell lines and tissues was subjected to RT-PCR and differential expression analysis of TRAF2 splice variants was carried out. RESULTS: An amplification of about 1 500 bp was found using P1 and P2 as the primers. we performed PCR using exon 6 flanking primers P3 and P4, yielding a full-length transcript of 268 bp ...