期刊论文

Effects of preconditioning with serum containing Xintongshu on the newborn rat＇s myocardial cells with hypoxia and reoxygenation injury and the expression of TNF-αand IL-Iβ

中文

湖南中医药大学学报

1674-070X

2012

32

11

11-15

10.3969/j.issn.1674-070X.2012.11.003.011.05

国家自然科学基金资助项目（30973750）； 湖南省教育厅重点科研项目（09A071）； 湖南省重点学科中医内科学资助项目；

本校为第一机构

目的 观察心痛舒含药血清预处理对缺氧/复氧乳鼠心肌细胞核因子-κBp65 (NF-κBp65)及其下游调控因子肿瘤坏死因子-α(TNF-α)、白介素-1β(IL-1β)的影响,探讨心痛舒对心肌细胞缺氧/复氧损伤的保护作用机制.方法 原代培养SD乳鼠心肌细胞,建立模拟心肌细胞缺氧/复氧损伤模型,设正常组、模型组、阳性对照组、心痛舒含药血清预处理组共4组.罗丹明B染色法观察心肌细胞形态,ELISA法测定培养液上清TNF-α、IL-1β含量,半定量RT-PCR方法检测NF-κBp65 mRNA表达水平.结果 与模型组比较,阳性对照组、心痛舒含药血清预处理组TNF-α、IL-1β水平降低,NF-κBp65 mRNA的表达水平下降,差异有统计学意义(P＜0....

Objective To observe the regulating effects of preconditioning with serum containing Xintongshu on the expression of nuclear factor-KBp65 （NF-KBp65）, tumor necrosis factor-α（TNF-α） and interleukin-lβ（IL-lβ）, and to explore the protective mechanism of Xintongshu on the hypoxia and reoxygen injury myocardial cell model of newborn rat. Methods The myocardial cells were isolated from neonatal rats and cultured,then modelled with hypoxia andreoxygenation injury. The modeled myocardial ceils were randomly divided into tour groups, mcmo ing ...