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Eps8 regulates cellular proliferation and migration of breast cancer

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成果类型:
期刊论文
作者:
Chen, Cheng;Liang, Zhongheng;Huang, Wenhuan;Li, Xinxin;Zhou, Fangliang(周芳亮);...
通讯作者:
Ding, XF
作者机构:
[Li, Xinxin; Ding, Xiaofeng; Chen, Cheng; Hu, Xiang; Liang, Zhongheng; Han, Mei; Huang, Wenhuan; Xiang, Shuanglin] Hunan Normal Univ, Key Lab Prot Chem & Dev Biol, State Educ Minist China, Coll Life Sci, Changsha 410081, Hunan, Peoples R China.
[Zhou, Fangliang] Hunan Univ Chinese Med, Coll Basic Med Sci, Changsha, Hunan, Peoples R China.
通讯机构:
[Ding, XF ] H
Hunan Normal Univ, Key Lab Prot Chem & Dev Biol, State Educ Minist China, Coll Life Sci, Changsha 410081, Hunan, Peoples R China.
语种:
英文
关键词:
EPS8 protein, human;signal transducing adaptor protein;small interfering RNA;adult;aged;animal;antagonists and inhibitors;Bagg albino mouse;breast tumor;cell motion;cell proliferation;drug effects;female;gene expression regulation;genetic transfection;genetics;human;MCF 7 cell line;medullary carcinoma;middle aged;mouse;nude mouse;Paget nipple disease;pathology;physiology;tumor cell culture;very elderly;Adaptor Proteins, Signal Transducing;Adult;Aged;Aged, 80 and over;Animals;Breast Neoplasms;Carcinoma, Ductal, Breast;Carcinoma, Medullary;Cell Movement;Cell Proliferation;Female;Gene Expression Regulation, Neoplastic;Humans;MCF-7 Cells;Mice;Mice, Inbred BALB C;Mice, Nude;Middle Aged;RNA, Small Interfering;Transfection;Tumor Cells, Cultured
期刊:
INTERNATIONAL JOURNAL OF ONCOLOGY
ISSN:
1019-6439
年:
2015
卷:
46
期:
1
页码:
205-214
基金类别:
National Natural Science Foundation of China [81272318, 81272190]; Scientific Research Fund of Hunan Provincial Education Department [13B068]
机构署名:
本校为其他机构
院系归属:
医学院
摘要:
The role of Eps8 in human breast cancer was studied, and we found that Eps8 was overexpressed in >60% of human breast cancer samples compared with adjacent normal breast tissues by immunohistochemical analysis. Eps8 was highly expressed in the highly invasive breast cancer cell line MDA-MB-231 compared with the weakly invasive breast cancer cell lines MCF7 and MDA-MB-468. MCF7 cell line stably expressing Eps8 was established by G418 screening, and the ectopic expression of Eps8 enhanced MCF7 breast cancer cell growth and survival as assessed by MTT analysis, cell viability and liquid colony fo...

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