期刊论文

Effect of Ganluxiaodudan on Coxsackievirus A16 mouse

中文

中药药理与临床

1001-859X

2017

33

2

13-18

10.13412/j.cnki.zyyl.2017.02.004

国家自然科学基金资助项目（81173188）；

本校为第一机构

目的:探讨甘露消毒丹对柯萨奇病毒A16型(CoxA16)小鼠模型的影响。方法: 7日龄ICR小鼠150只,随机分为正常组、模型组、阿昔洛韦组、甘露消毒丹组,每组25只;除正常组外,其余各组每只小鼠腹腔内注射20ul 10~7 TCID_(50)的CoxA16标准株原液进行造模,造模后的第5天,除正常组和模型组外,其余4组使用相应的药物干预治疗。观察小鼠临床表现;在光镜下观察肌肉组织形态学变化;用细胞增殖与毒性检测试剂盒(CCK-8)检测肌肉匀浆液对Hela细胞存活率的变化;电镜观察肌肉组织病毒颗粒的变化,采用ELISA法检测肌肉组织IL-6、IL-10、INF-γ的浓度。结果:与模型组比较,发现阿昔洛韦及甘露消毒丹(24.4g /kg、12.2g /k...

Objective：To study the effect of Ganluxiaodudan on Coxsackievirus AI 6 （CoxAl 6） mouse. Methods： 150 ICR mice at 7 days old were randomly divided into the control group, the model group, acyclovir group and mannose disinfectant group （ n = 25 ）. Except the control group, mice in each group were intraperitoneally injected into 20μl 10^7 TCID50 CoxA16 standard stock solutionto establish the model. On the fifth day after modeling, other four groups were treated with the same medicine except the control group and the model group. The morphol...