期刊论文

Effect of Berberine on Intestinal Flora of Obese Mice and Its Mechanism

中文

基因组学与应用生物学

1674-568X

2019

38

2

810-814

10.13417/j.gab.038.000810

本校为第一机构

为了探索黄连素对肥胖小鼠肠道菌群的影响和作用机制,本研究将40只雄性昆明种小鼠随机分为空白组、模型组、黄连素高剂量组、黄连素中剂量组和黄连素低剂量组,每组8只,除空白组外,其余4组给予高脂饮食,建立实验性肥胖小鼠模型。造模14 d后,全部给予正常饮食,黄连素高、中、低剂量组灌胃给予每天0.1~0.3 mL/10 g的药物干预,空白组和模型组给予等剂量的生理盐水,给药持续14 d。每周称量两次体质量,并分别于实验第0、14、28天从眼眶取血测定血脂和炎性因子的含量,收集小鼠粪便测定乳酸杆菌和双歧杆菌的数量。研究结果表明:空白组和模型组小鼠的体质量在第14天时有明显差异,造模成功;实验第28天,黄连...

In order to explore the effect and action mechanism of berberine on intestinal flora of obese mice, 40 male Kunming mice were randomly divided into the control group (CN), the control model group (CM), the berberine high-dose group (BH), the berberine medium dose group (BM), and the berberine low dose group (BL), with 8 mice in each group. In addition to the blank group, the other 4 groups were given high-fat diet to establish an experimental obese mouse model. After 14 days of modeling, normal diet was given to all, and 0.1~0.3 mL/10 g of drug intervention was given by the gastric lavage to t...