期刊论文

Effect of NF-κB Signal on Osteoclast Differentiation of Staphylococcus aureus and Its Role

中文

基因组学与应用生物学

1674-568X

2020

39

04

1837-1844

10.13417/j.gab.039.001837

本校为第一机构

为探讨金黄色葡萄球菌对破骨细胞分化的影响和可能的作用机制,本研究采用活的金黄色葡萄球菌、灭活的金黄色葡萄球菌和金黄色葡萄球菌滤液处理RAW 264.7细胞5 d。观察破骨细胞样细胞和吸收凹坑的形成,并通过实时定量PCR检测破骨细胞特异性基因（TRAP, MMP-9,组织蛋白酶K, CTR和Atp6v0d2）的表达。此外,用蛋白质印迹法检测了与破骨细胞分化有关的信号通路中的关键蛋白。结果显示,灭活的金黄色葡萄球菌的感染复数和金黄色葡萄球菌滤液以及活的金黄色葡萄球菌（MOI为10∶1 CFU）对RAW 264.7细胞没有明显的细胞毒性。当没有RANKL时,活的金黄色葡萄球菌、灭活的金黄色葡萄球菌和金黄色葡萄球菌滤液以...

To investigate the effect of Staphylococcus aureus on osteoclast differentiation and its possible molecular mechanism.RAW 264.7 cells were treated with live Staphylococcus aureus,inactivated Staphylococcus aureus and Staphylococcus aureus filtrate for 5 days.Then,the formation of osteoclast-like cells and absorption pits was observed,and the expression of osteoclast-specific genes (TRAP,MMP-9,cathepsin K,CTR and Atp6v0d2) was detected by Real-time quantitative PCR.In addition,key proteins in the signaling pathways involved in osteoclast differe...