期刊论文

Experimental study on the apoptosis of human gastric cancer BAC823 cells induced by β-elemene

中文

中南药学

1672-2981

2005

3

6

329-331

10.3969/j.issn.1672-2981.2005.06.003

湖南省自然科学基金项目（项目编号:00JJY2033）；

本校为其他机构

目的探讨β-榄香烯对人胃癌BAC823细胞的增殖抑制作用及其对细胞凋亡的影响.方法每批细胞按空白对照组和药物处理组随机分组,其中药物处理组依药物浓度的不同再分为0.01、0.02、0.04、0.08、0.10、0.16 mg·mL-1处理组,作用时间为24 h.采用MTT法检测β-榄香烯对癌细胞的增殖抑制作用;采用流式细胞光度分析术(FCM)检测细胞凋亡率及细胞周期时相分布,同时进行细胞的形态学观察.结果①MTT的检测结果显示药物浓度和细胞增殖抑制率间具有正相直线相关关系(P＜0.01),β-榄香烯体外作用24 h,对胃癌细胞的IC50为0.078 mg·mL-1.②FCM检测及细胞形态学观察结果显示β-榄香烯可阻滞BAC823细胞于S期并诱导细...

OBJECTIVE To explore the effect of β-elemene on the inhibition of proliferation and apoptosis of human gastric cancer BAC823 cells. METItODS The cells were divided into two groups： the blank control group and the drug group. The drug group was divided into 0. 01, 0. 02, 0. 04, 0.08, 0.10 and 0.16 mg · mL^-1 groups. The duration was 24 h. Inhibition of proliferation was measured with MTT assay. Apoptotic rate and fraction of the cell cycle phase were measured with flow cytometry. At the same time, morphological and adhesive changes of the cel...