期刊论文

中文

中医药学报

1002-2392

2003

31

5

60-61

10.19664/j.cnki.1002-2392.2003.05.033

30171174,30271678,39770933:国家自然科学基金 00JJY2031:湖南省自然科学基金 01A014;2001]98;0109:湖南省教育厅科学研究项目 2000014:湖南省中医药局科研项目

本校为第一机构

目的:观察气虚证模型大鼠鼻咽组织B区基因表达谱特征及其对治疗的反应.方法:药物加疲劳法在Wistar大鼠制作气虚证模型,随机分为治疗组与对照组,以AtlasTMcDNA阵列法检测各组动物鼻咽组织B区基因表达谱,比较分析其表达谱差异.结果:模型组动物表现Ⅰ-kBα链、MAPK1、MAPK2、GNB2基因表达上调,MEK5、PLCδ、RGP(Rab相关GTP结合蛋白)基因表达下调;治疗1周后,表达上调的MAPK1、MAPK2二基因活性恢复正常,其余5个基因表达活性仍维持治疗前活性状态.结论:单纯气虚证动物出现调制子、效应子和细胞内转导子基因表达活性异常,细胞内信号传导通路发生障碍.经针对性治疗后,这些障碍可以逐渐恢复正常.

Objective:To investigate the features of gene expression profilingon B area of Atlas TM cDNA array and their respones to treatment in treatment in nasopharyngeal tissues among rats with Qi deficiency.Methods:Qi deficiency was induced among rats with the method of herbal decoction perfused gastrically and supplemented by forced swimming in deep pool.The expressive features of genes on B area were determined in nasopharyngeal tissues of tested rats on Atlas TM cDNA expression array.Results:The genes showing up regulated expressive activities incl...