期刊论文

Effect of Xiaochaihu Decoction on Pulmonary Inflammation Induced by NTHi

中文

中医药学报

1002-2392

2019

47

4

28-32

10.19664/j.cnki.1002-2392.190106

2018-01:湖南中医药大学大学生研究性学习与创新性实验计划 2018-31:湖南中医药大学大学生研究性学习与创新性实验计划 2018-47:湖南中医药大学大学生研究性学习与创新性实验计划 2018-412:湖南省大学生研究性学习和创新性实验计划

本校为第一机构

目的:探讨小柴胡汤干预不可分型嗜血流感杆菌(NTHi)诱导肺部炎症的药效机制。方法:①建立NTHi感染小鼠模型,实验同步设生理盐水(NS)组、NTHi组、氨苄西林(Amp)组和小柴胡汤(XCH)组,灌胃给予药物7天后,处理动物,进行指标检测;②检测小鼠体重、肺指数及免疫器官指数;③HE染色观察小鼠肺组织病理变化;④ELISA法检测小鼠血清中IL-1β、IL-6、GCSF、TNF-α含量水平。结果:①与NC组比较,NTHi组小鼠精神萎靡,不好动,毛发竖起,皮肤松弛,经常蜷缩在一起,饮食量少;与NTHi组比较,经小柴胡汤或氨苄西林药物干预后,小鼠状态得到不同程度地缓解;②HE染色观察表明:小柴胡汤和氨苄西林均能不同程度改善由NTHi干预...

Objective: To investigate the mechanism of Xiaochaihu Decoction (XCD) intervening pulmonary inflammation induced by non-phenotype Haemophilus influenzae (NTHi). Methods: The mice model of NTHi infection was established, and randomly divided into the NTHi group, Ampicillin group and Xiaochaihu Decoction group, the normal mice were taken as the normal control. After seven days of medication, body weight, lung index and immune organ index were measured, the pathological changes of lung tissue were observed by HE staining method. The serum levels o...