期刊论文

中文

湖南中医药大学学报

1674-070X

2011

31

9

12-15

10.3969/j.issn.1674-070X.2011.09.003.012.04

国家自然科学基金资助项目（30772696,30973717）； 湖南省研究生创新基金（CX2009B168）；

本校为第一机构

目的 观察骨髓间充质干细胞(MSCs)移植于大鼠缺血心肌后,心脏早发育基因GATA-4mRNA的表达.方法 设模型组、假手术组和空白对照组,其中模型组采用“冠脉结扎法”建立大鼠急性心肌梗死(AMI)模型.收集从SD雄性大鼠胫骨和股骨骨髓中分离、培养、鉴定后的MSCs,密度调至3x106/100 μL悬液,移植于缺血心肌边缘组织的3个位点直接注入共100 μL的MSCs细胞悬液,假手术组和空白对照组均在与模型组相同注射部位的3个位点直接注入共100 μL的MSCs细胞悬液,28 d后处死大鼠进行相关实验样本处理.采用逆转录聚合酶链反应(RT-PCR)技术检测心肌组织中GATA-4mRNA的表达.结果MSCs移植模型组和假手术组的大鼠心肌中存...

Objective To observe the expression of genes GATA-4 mRNA in the early heart development after bone marrow mesenchymal stem cells（MSCs） were transplanted into ischemic myocardium.Methods The model group,sham group and control group were set,and the acute myocardial infarction（AMI） model was established according to ＂coronary occlusion＂ method.,MSCs were collected the isolated,cultured and identified marrow from the tibia and femur of SD male rats,the density of MSCs was adjusted to 3×106/100 μL and then to be transplanted into three poin...