期刊论文

Effects of Danlong Xingnao recipe on the proliferation of neural stem cells in hippocampus of rats with cereral ischemiareperfusion injury and the expressions of Frizzled,Dvl,CyclinD1

中文

中药药理与临床

1001-859X

2016

32

1

120-124

10.13412/j.cnki.zyyl.2016.01.034

国家自然科学基金（81373702、81473567、81202632） 教育部博士点基金（20124323120003） 湖南省自然科学基金（13JJ3097） 湖南省教育厅科研项目（14B134,15K092）

本校为第一机构

目的：探讨丹龙醒脑方对局灶性脑缺血再灌注模型大鼠海马区神经干细胞增殖,以及Frizzled1、Dvl1、Cyclin D1表达的影响。方法：将80只雄性SD大鼠随机分为假手术组、模型组、依达拉奉组、丹龙醒脑方7.4g/kg组和丹龙醒脑方14.8g/kg组,采用线栓法制备局灶性脑缺血再灌注模型,再灌注1d、3d和7d采用改良m-NSS法进行神经功能缺损评分,7d后取大鼠缺血侧海马组织,采用免疫荧光法检测海马区Brdu阳性细胞率;采用RT-q PCR法、Western Blot法分别检测Frizzled1、Dvl1、Cyclin D1 mRNA和蛋白表达。结果：1再灌注1d,与假手术组比较,其余各组神经功能缺损评分明显升高,而各组间差异无统计学意义;再灌注3d、7d,与...

Objective: To investigate the effects of Danlong xingnao recipe on proliferation of neural stem cells (NSCs) in hippocampus and the expressions of Frizzled1,Dvl1,CyclinD1 with cerebral ischemia-reperfusion injury modle rats. Methods: 80 male SD rats were randomly divided into sham group,cerebral ischemia-reperfusion injury (model) group,edaravone group and Danlong Xingnao recipe (DLXNR) 7.4g /kg group,DLXNR14.8g /kg group. The focal cerebral ischemia reperfusion injury models were prepared by suture method,1d,3 d, and 7d after reperfusion, cond...