期刊论文

中文

中国动脉硬化杂志

1007-3949

2013

21

8

700-704

湖南省教育厅基金（09C845）； 湖南省高校创新平台开放基金（12K096）； 南华大学研究生科研创新项目（2011XCX22）资助；

本校为其他机构

建立兔颈总动脉血管吻合口狭窄模型,并用局部转染小凹蛋白(Caveolin-1)质粒,观察血管吻合口部位Caveolin-1对白细胞介素10( IL-10)和白细胞介素6 (IL-6)表达的影响。方法从南华大学动物中心取成年家兔32只,行颈总动脉血管吻合术,运用脂质体局部转染Caveolin-1质粒,随机分为4组:正常组(假手术组)、手术组、手术+空转染组、手术+转染组。分别于7天及28天取颈总动脉标本,通过HE染色观察内膜增生情况,并测量内膜与中膜面积比值(IA/MA);实时荧光定量PCR检测血管中Caveolin-1和IL-6、IL-10 mRNA的表达,免疫组织化学观察血管组织中IL-6、IL-10蛋白的表达。结果HE染色显示,手术组及空转染组内膜明显增殖...

Aim To investigate the effect of the caveolin-I on the expression of intedeukin-10 （IL-10） and inter- leukin-6 （IL-6） in rabbit carotid artery anastomotic stoma. Methods 32 New Zealand white rabbits were randomly di- vided into sham operation group, operation group, empty vector group and caveolin-1 transfected group. The rest speci- mens were taken for HE staining at 7 d and 28 d, which were used to observe the proliferation of intima, and the ratio of in- tima/media area was measured by Image-Pro Plus 6. 0 software ; Real time PCR was use...