[Objective] To obtain the optimum reflux extraction conditions for toosendanin from FRUCTUS TOOSENDAN.[Method] Contetn of toosendanin was determined by HPLC,and effects of extraction solvent,solid to liquid ratio,extraction temperature,extraction duration and extraction times on yield of toosendanin were investigated using content of toosendanin as index.[Result] The optimal extraction conditions for extracting toosendanin by reflux extraction were proposed to be: extraction solvent of methanol,solid to liquid ratio of 1∶30,extraction tempera...