期刊论文

Effect of Bushen Huoxue decoction on expression of cyclooxygenase-1 mRNA in PCO rats

中文

湖南中医药大学学报

1674-070X

2009

29

4

18-20

10.3969/j.issn.1674-070X.2009.04.006

湖南省自然科学基金资助项目（06JJ50067）； 湖南省教育厅（青年）基金资助项目（05B035）；

本校为第一机构

目的 探讨环氧化酶(COX-1)的表达在多囊卵巢卵泡破裂中的作用及补肾活血方治疗多囊卵巢排卵障碍的作用机制.方法 选用未成年24 d龄SD雌性大鼠60只,随机分为模型组、克罗米酚对照组、补肾活血方高剂量组、补肾活血方低剂量组、正常对照组5组,每组12只.用Bogovich法建立大鼠多囊卵巢病理模型,以克罗米酚为对照,采用原位杂交法检测各组卵巢组织中环氧化酶COX-1基因的表达水平.结果 模型组与正常组比较,卵泡内膜细胞COX-1mRNA表达明显增加,两者比较差异有统计学意义(P0.05).结论 COX-1mRNA在卵巢卵泡内膜细胞水平升高可能是PCOS发病的重要因素或环节.补肾活血方可能通过影响COX-1mRNA水平,从而发挥治...

Objective To investigate the effect of COX-1 expression in ovary cell and the meehanism of Bushen Huoxue decoetion （BHD） in PCO rats. Methods 60 SD female rats of 24 days age were randomly divided into high dosage group of BHD, low dosage group of BHD, model group, western medicine group and normal control group, 12 rats per group. Chloramiphene used to be controlled and the models induced with Bogovieh method, the COX- 1 expression was detected. Results Compared with normal eontrol group, the expression of COX-1 mRNA in model group was inere...