期刊论文

The Effects of Zuo Gui Shuang Jiang Fang on ICAM-1, tPA, and PAI of the Vascular Endothelial Cells Injured by Glucose, Insulin and Low-Density Lipoprotein

中文

中华中医药学刊

1673-7717

2005

23

1

61-63

10.13193/j.archtcm.2005.01.59.yur.028

湖南省教育厅青年基金

本校为第一机构

目的:观察左归双降方提取液对葡萄糖(Glu)、胰岛素(Ins)、氧化低密度脂蛋白(ox-LDL)联合诱导下血管内皮细胞损伤的干预作用与作用机理.方法:以人脐带静脉内皮细胞株ECV-304为受试对象,以不同浓度的Glu、Ins、ox-LDL联合作用24h,建立了一种糖尿病血管并发症的体外内皮细胞损伤模型;并在此基础上观测左归双降方提取液(ZGSJF)对该细胞损伤模型的调节作用.各试验组细胞继续培养48h后分别检测细胞活性及其细胞培养液中的细胞黏附因子(ICAM-1)、组织型纤溶酶原激活物(tPA)、纤溶酶原激活物抑制物(PAI)的含量.结果:细胞继续培养48h后,模型组较正常组细胞活力明显下降(P＜0.01),PAI及ICAM-1含量明显升高...

Objective :To investigate the effects of Zuoguishuangjiangfang(ZGSJF) on vascular endothelial cells injured by glucose,insulin and low density lipoprotein. Methods :We made human umbilical vein endothelial cell line ECV 304 exposed to different concentration of glucose,insulin and oxidized low density lipoprotein(Ox LDL)for 24 hours,and to investigate the effects of ZGSJF on this injured vascular endothelial cells.The cells in each group were cultured for additional 48 hours.And then,cell viability was examined,and the supernatants were used to...