期刊论文

中文

中国临床解剖学杂志

1001-165X

2011

29

5

546-549

10.13418/j.issn.1001-165x.2011.05.020

国家自然科学基金（81070897）；

本校为其他机构

目的探讨完全弗氏佐剂（CompleteFreund’sAdjuvant,CFA）致大鼠炎性疼痛后海马区CREB结合蛋白（CREB Binding Protein,CBP）的表达变化及意义。方法大鼠随机分为正常组和实验组,实验组大鼠左侧足底皮下注射CFA和生理盐水混合溶剂100μl,建立炎性疼痛模型,分为注射CFA后6 h、1 d、3 d、7 d和14 d组。采用Von Frey纤维观察不同时间点大鼠机械缩足阈值（Paw withdrawalthreshold,PWT）的变化;采用免疫组化法检测海马CBP的表达变化。结果足底注射CFA后1 h PWT即下降,并在6 h后达到最低值,3 d后逐渐上调,至14 d仍低于基础值。正常组大鼠海马各区均可见CBP大量表达。实验组大鼠两侧海马各区尤其是CA1...

Objective To explore expression changes of CBP（CREB Binding Protein） in rat hippocampus followed by Complete Freund＇s Adjuvant（CFA） induced inflammatory pain.Methods Rats were received plantar subcutaneous injection of CFA and saline mixed solvent 100ul,for establishing inflammatory pain model.Model rats were randomly divided into normal group,CFA 6h,1d,3d,7d and 14d groups.The change of paw withdrawal threshold（PWT） was observed by Von Frey fibers in rats at different time points;the expression of CBP was detected using immunohistochemi...