期刊论文

Preliminary study on pyroptosis involved in cerebral ischemia-reperfusion injury

中文

中国病理生理杂志

1000-4718

2019

35

8

1379-1386

10.3969/j.issn.1000-4718.2019.08.006

国家自然科学基金资助项目(No.81603415) 湖南省自然科学基金资助项目(No.2017JJ3231) 湖南省中医药管理局重点项目(No.201820) 湖南省教育厅科研项目(No.15C1034) 2016年度湖南省大学生研究性学习和创新性实验计划(No.959).

本校为第一机构

目的:探讨细胞焦亡在脑缺血再灌注后不同时点海马和皮质区的变化,从NLRP3炎症小体介导的经典焦亡途径探讨其机制,分析细胞焦亡在脑内不同部位损伤中的作用。方法: SD大鼠随机分成假手术组( sham组)与模型组( MCAO/R组),模型组又分为脑缺血再灌注6 h组( MCAO/R 6 h组) 、12 h组( MCAO/R 12 h组)和24 h组( MCAO/R 24 h组) 。右侧改良线栓法制备大鼠大脑中动脉缺血-再灌注模型。采用神经功能评分、 2,3,5-氯化三苯基四氮唑( TTC)染色法和形态学观察评价神经细胞损伤程度; TUNEL和caspase-1免疫荧光双染检测细胞焦亡; Western blot法检测NLRP3、cleaved caspase-1、pro-caspase-1和白细胞介素1β( I...

AIM: To investigate the changes of pyroptosis in hippocampus and cortex at different time points after cerebral ischemia-reperfusion,and to explore its mechanism from NLRP3-mediated classical pyroptosis pathway,and to analyze the role of pyroptosis in different parts of cerebral injury. METHODS: SD rats were randomly divided into sham operation group ( sham group) and model group ( MCAO/R group). The rats in model group was further divided into cerebral ischemia-reperfusion 6 h group ( MCAO/R 6 h group),12 h group ( MCAO/R 12h group) and 24 h g...