期刊论文

Effect of Jiawei-Naotai formula on ATF4 /CHOP /Puma pathway of ovariectomized rats with cerebral ischemia

中文

中国病理生理杂志

1000-4718

2019

35

2

365-369

10.3969/j.issn.1000-4718.2019.02.028

国家自然科学基金资助项目(No.81603604) 湖南省自然科学基金资助项目(No.2018JJ3390) 湖南省教育厅资助科技项目(No.17B196) 湖南省中医药科研计划项目(No.201784) 湖南省高校创新平台项目(No.17k072) 长沙市科技计划项目(No.K91801045)。

本校为第一机构

目的:研究加味脑泰方对去卵巢脑缺血大鼠海马活化转录因子4 (ATF4) /C/EBP同源蛋白 (CHOP) /p53上调凋亡调控因子 (Puma) 通路的影响.方法:将雌性大鼠随机分为假手术组、模型组、加味脑泰方组和阳性对照组, 除假手术组外, 其它大鼠均行去卵巢术;术后11 d, 阳性对照组和加味脑泰方组给予相应药物灌胃, 连续灌胃3 d后行脑缺血术.术后24 h进行行为学评价, 取海马组织行RT-qPCR检测Bax、Bcl-2和caspase-3的mRNA表达, Western blot检测各组大鼠海马组织中Bax、Bcl-2、caspase-3、ATF4、CHOP和Puma蛋白表达.结果:与假手术组比较, 各组的海马神经功能评分明显增高;与模型组比较, 加味脑泰方组与阳性对照...

AIM: To investigate the effects of Jiawei-Naotai formula ( JWNTF) on ATF4 /CHOP/Puma pathway in hippocampal neurons of ovariectomized female rats with cerebral ischemia. METHODS: The female rats were randomly divided into sham group,model group,JWNTF group and positive control group. The rats,expect in the sham group,were ovariectomized. The rats in each group were intragastric administration 11 days after ovariectomy. The rats in sham group and model group were given a gavage of 0. 9% NaCl,while the rats in other groups were administrated by corresponding therapy intragastrically for 3 d. The...