期刊论文

Effect of Kechuanning on airway remodeling and protein level of p-ERK1/2 in lung tissues of asthmatic rats induced by virus

中文

中国病理生理杂志

1000-4718

2019

35

7

1268-1275

10.3969/j.issn.1000-4718.2019.07.019

国家自然科学基金资助项目(No.81674025 No.81774368) 湖南省自然科学基金资助项目(No.2015JJ2016) 湖南中医药大学大学生研究性学习和创新性实验课题计划(No.2018-16).

本校为第一机构

目的:探讨咳喘宁对病毒诱发哮喘大鼠气道重塑及肺组织p-ERK1/2蛋白表达的影响。方法:构建呼吸道合胞病毒感染诱发哮喘建立大鼠哮喘模型,实验分为正常组、哮喘模型组、咳喘宁低剂量(0. 33 mL/kg)组、咳喘宁中剂量(3 mL/kg)组、咳喘宁高剂量(10 mL/kg)组及PD98059(3 mg/kg)组;采用动物呼吸机测量大鼠气道反应性; HE染色法观察肺组织的病理形态学改变; PAS染色法和Masson染色法观察杯状上皮化生和气道胶原沉积情况。免疫组织化学染色法检测大鼠肺组织中基质金属蛋白酶9(MMP-9)和基质金属蛋白酶组织抑制因子1 (TIMP-1)的表达; Western blot法检测大鼠肺组织中的ERK1/2和p-ERK1/2表达。结果:与模型组...

AIM: To investigate the effect of Kechuanning on airway remodeling and the protein level of p- ERK1/2 in lung tissues of asthmatic rats induced by virus. METHODS: The asthmatic rat model induced by respiratory syncytial virus was established. The experimental rats were divided into normal group,asthma model group,low dose (0. 33 mL/kg),middle dose (3. 0 mL/kg) and high dose (10 mL/kg) of Kechuanning groups,and PD98059 (3 mg/kg) group. The airway responsiveness of the rats was measured by animal ventilator. The pathological changes of the lung tissues were observed by HE staining. PAS staining ...