期刊论文

Effects of Anhua dark tea on ApoE~(–/–) mice with non-alcoholic fatty liver induced by high-fat diet

中文

中国病理生理杂志

1000-4718

2020

36

7

1274-1280

10.3969/j.issn.1000-4718.2020.07.018

湖南省科技计划项目(No.2018JJ2412) 湖南省中医药科研计划项目(No.201870) 湖南省教育厅科学研究项目(No.17B271)。

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目的:探讨安化黑茶对高脂饮食诱导的载脂蛋白E敲除(ApoE~(–/–))小鼠非酒精性脂肪肝的防治作用及相关机制。方法:选取8周龄雄性ApoE~(–/–)小鼠50只,随机分为模型组、阿托伐他汀组和安化黑茶高、中、低剂量组,每组10只,另选用10只8周龄C57BL/6J雄性野生小鼠作为正常对照组,ApoE~(–/–)和野生小鼠分别予以等量高脂饲料和普通饲料持续喂养17周,同时用相应药物或生理盐水灌胃干预。实验末取部分肝组织行HE染色,并检测丙氨酸转氨酶(ALT)、天冬氨酸转氨酶(AST)、超氧化物歧化酶(SOD)和谷胱甘肽过氧化物酶(GSH-Px)活性及丙二醛(MDA)含量;RT-qPCR检测羟甲基戊二酸单酰辅酶A还原酶(HMGCR)、过氧化物酶...

AIM:To explore the effects of Anhua dark tea on the prevention and treatment of non-alcoholic fatty liver induced by high-fat diet in apolipoprotein E knockout(ApoE~(–/–))mice and the relevant mechanisms.METHODS:Male ApoE~(–/–) mice(n=50,8 weeks old)were randomly divided into model group,atorvastatin group,and high-,mediumand low-dose Anhua dark tea groups,with 10 mice in each group.In addition,10 homologous wild-type male C57BL/6J mice were selected as normal control group.The ApoE~(–/–) and wild-type mice were fed with the same amount o...