期刊论文

Immunomodulatory effect of Sema4D on Th1/Th2 in asthmatic rats in- duced by RSV and intervention of Kechuanning oral liquid

中文

中国病理生理杂志

1000-4718

2020

36

8

1465-1469

10.3969/j.issn.1000-4718.2020.08.018

No.81674025:国家自然科学基金 No.81774368:国家自然科学基金 :湖南中医药大学中医学国内一流建设学科

本校为第一机构

目的:探讨脑信号蛋白4D(semaphorin 4D,Sema4D)对呼吸道合胞病毒(respiratory syncytial virus, RSV)所致哮喘大鼠Th1/Th2的免疫调控及咳喘宁的干预作用。方法:60只SPF级Wistar大鼠随机分为10组,即正常组,模型组,Sema4D组,Sema4D抗体组,咳喘宁低、中、高剂量组,以及Sema4D+咳喘宁低、中、高剂量组。除正常组外,其余各组采用RSV合并卵清蛋白(ovalbumin,OVA)诱导大鼠哮喘模型。HE染色观察肺组织病理变化; ELISA法检测支气管肺泡灌洗液(bronchoalveolar lavage fluid,BALF)中炎症因子白细胞介素4(interleukin-4,IL- 4)、IL-6、IL-8、肿瘤坏死因子α(tumor necrosis factor-α,TNF-α)和干扰素γ(in...

AIM:To explore the effect of semaphorin 4D(Sema4D)on the immune regulation of Th1/Th2 in asthmatic rats induced by respiratory syncytial virus(RSV)and the effect of Kechuanning(KCN)intervention on the asthmatic rats. METHODS:Healthy SPF female Wistar rats(n=100)were randomly divided into 10 groups:normal group, model group,Sema4D group,Sema4D antibody group,low,middle and high doses of KCN groups,and Sema4D+low, middle and high doses of KCN groups. Except the rats in normal group,the other rats were treated with RSV combined with ovalbumin(OVA)...