期刊论文

Panax notoginseng saponins suppress oxygen-glucose deprivation/reoxygenation-induced pyroptosis of SH-SY5Y cells

中文

中国病理生理杂志

1000-4718

2020

36

7

1178-1184

10.3969/j.issn.1000-4718.2020.07.004

国家自然科学基金资助项目(No.81503385) 2019年湖南省教育厅科学研究项目优秀青年项目(No.19B436) 2018年湖南省大学生创新创业训练计划项目(No.433) 2018年度湖南中医药大学中西医结合一流学科开放基金项目(No.2018ZXYJH35) 湖南中医药大学“十三五”一级学科基础医学建设项目(No.06)。

本校为第一机构

目的:探讨三七总皂苷(PNS)对缺氧缺糖/复氧复糖(OGD/R)诱导的SH-SY5Y细胞焦亡的调控作用。方法:以OGD/R诱导构建体外缺血再灌注SH-SY5Y细胞模型,观察PNS对SH-SY5Y细胞活力(CCK-8法)及细胞膜通透性[表示为乳酸脱氢酶(LDH)漏出率和碘化丙啶(PI)染色阳性细胞比例]的影响,分析PNS对细胞中gasdermin D(GSDMD)、GSDMD N端片段(GSDMD-N)、caspase-1和caspase-4蛋白水平变化以及白细胞介素1β(IL-1β)和IL-18释放的影响。结果:OGD/R诱导的SH-SY5Y细胞活力显著降低(P

AIM:To investigate the effect of Panax notoginseng saponins(PNS)on pyroptosis of SH-SY5 Y cells induced by oxygen-glucose deprivation/reoxygenation(OGD/R).METHODS:The OGD/R was conducted to induce ischemia/reperfusion injury in SH-SY5 Y cells.The effects of PNS on the viability(detected by CCK-8 assay)and membrane permeability[indicated by lactate dehydrogenase(LDH)leakage and propidium iodide(PI)staining positive cell proportion]of OGD/R-induced SH-SY5 Y cells were observed.The protein levels of gasdermin D(GSDMD),GSDMD Nterminal fragment(GSDM...