期刊论文

Evaluation of methods for establishing a mouse model of type 2 diabetes mellitus combined with atherosclerosis

中文

中国病理生理杂志

1000-4718

2021

37

07

1338-1344

10.3969/j.issn.1000-4718.2021.07.026

国家自然科学基金资助项目（No.81573956;No.82074400）； 国家重点研发计划中医药现代化研究重点专项（No.2018YFC1704300）； 湖南省重点研发计划项目（No.2020SK2101）； 湖南省教育厅科研项目（No.18A220;No.20K094）；

本校为第一机构

目的:对比2型糖尿病合并动脉粥样硬化的造模方法,研究优化模型条件,构建一种发病过程类似人类2型糖尿病合并动脉粥样硬化的小鼠模型。方法:8周龄MKR小鼠20只,随机选取10只作为MKR空白组,剩余MKR小鼠(MKR模型组)与FVB/N小鼠(FVB/N对照组,10只)及ApoE^(-/-)小鼠(ApoE^(-/-)模型组,10只)腹腔注射链脲佐菌素(STZ),后经高脂乳剂及N-硝基-L-精氨酸甲酯(L-NAME)灌胃联合诱导完成糖尿病动脉粥样硬化模型制备,另取10只FVB/N小鼠作为正常组。造模诱导12周后采血,血糖仪(电化学法)检测空腹血糖;全自动生化分析仪检测血脂相关指标;化学发光法测定血清胰岛素水平;ELISA检测血清C-反应蛋白(CRP)、肿瘤坏死因子...

AIM:To evaluate methods for establishing diabetic atherosclerosis mouse model. METHODS: Twenty MKR mice were divided into 2 groups,MKR blank group and MKR model group. Intragastric gavage of fat emulsion and N-nitro-L-arginine-methyl ester(L-NAME)combined with streptozotocin(STZ)intraperitoneal injection was conducted in FVB/N control,MKR model and ApoE-/- model groups for establishing diabetic atherosclerosis model. Another 10 FVB/N mice with similar age and weight were used as normal group. After 12 weeks of modeling,the pathological changes ...