期刊论文

Effect of Zuogui-Jiangtang-Jieyu decoction on dendritic spines of hippocampal neurons in diabetic rats with depression via SIRT1/TORC1 signaling pathway

中文

中国病理生理杂志

1000-4718

2021

37

03

400-407

10.3969/j.issn.1000-4718.2021.03.003

国家自然科学基金资助项目（No.81874464）； 湖南省自然科学基金资助项目（No.2019JJ50464;No.2019JJ50345）； 湖南省卫生健康委科研课题（No.202102041447）； 湖南省中医药研究院重点项目（No.201803）；

本校为第一机构

目的:探明左归降糖解郁方调控糖尿病并发抑郁症大鼠海马SIRT1/TORC1信号通路并增加神经元树突棘的机制。方法:建立糖尿病并发抑郁症大鼠模型,并分别给予高、中、低剂量(20.52、10.26和5.13 g/kg)左归降糖解郁方。采用ELISA法测评各组大鼠血糖和胰岛素抵抗水平,采用强迫游泳实验和悬尾实验评价大鼠的抑郁样行为,采用Golgi-Cox染色观察海马神经元树突棘形态,采用Western blot法检测海马组织中SIRT1和TORC1蛋白表达。随后,给予模型大鼠海马脑区注射SIRT1的激动剂SRT2104和抑制剂sirtinol,研究SIRT1/TORC1信号通路影响树突棘形态的分子途径。最后,在给予高剂量左归降糖解郁方的基础上注射sirtinol,进...

AIM:To explore the mechanism of Zuogui-Jiangtang-Jieyu decoction increasing dendritic spines of hippocampal neuron in diabetic rats with depression via SIRT1/TORC1 signaling pathway.METHODS:The rats of diabetes complicated with depression were established and treated with high-,medium-and low-dose(20.52,10.26 and 5.13 g/kg)Zuogui-Jiangtang-Jieyu decoction.ELISA was used to measure the blood glucose and insulin resistance of the rats.The forced swimming test and the tail suspension test were used to evaluate the depression-like behavior.Golgi-Cox staining was used to observe the morphological c...