期刊论文

Influenza virus-induced lung epithelial cell injury and differential expression profile of miRNAs in exosomes

中文

中国病理生理杂志

1000-4718

2022

38

01

40-49

10.3969/j.issn.1000-4718.2022.01.006

国家自然科学基金资助项目（No.81973670;No.82074250）； 湖南省自然科学基金资助项目（No.2020JJ5418;No.2020JJ4063）； 中西医结合一流学科开放基金资助项目（No.2020ZXYJH54）；

本校为第一机构

目的:通过研究甲型流感病毒刺激后小鼠肺上皮细胞源性外泌体的微小RNA(miRNA)差异表达谱,探讨流感病毒诱导肺上皮细胞损伤的机制。方法:采用甲型流感病毒(MOI=0.2)诱导小鼠肺上皮MLE-12细胞,同时设空白对照组,通过CCK-8法检测不同诱导时间下的细胞活力,明确甲型流感病毒诱导细胞损伤的最佳条件。通过流式细胞术检测细胞凋亡水平,RT-qPCR和Western blot检测炎症反应相关因子的表达水平。采用超速离心法提取甲型流感病毒诱导的MLE-12细胞上清液中的外泌体,并通过透射电镜、纳米颗粒跟踪分析、Western blot及高通量单细胞测序检测外泌体形态、粒径、浓度、膜标志蛋白表达及其miRNA差异表达谱。结果...

AIM:To explore the mechanism of influenza virus-induced lung epithelial injury by studying microRNA( miRNA)expression profiles of lung epithelial cell-derived exosomes induced by influenza A virus.METHODS: Mouse lung epithelial MLE-12 cells were infected by influenza A virus(MOI=0.2),and a blank control group was set simultaneously. In order to determine the optimal intervention conditions for influenza A virus-induced cell injury,the cell viability at different time pionts was detected by Cell Counting Kit-8.The cell apoptosis was detected by ...