期刊论文

Li, S.

中文

中国中药杂志

1001-5302

1999

24

2

100-2, 126

10.3321/j.issn:1001-5302.1999.02.018

本校为第一机构

目的: 建立苦参中生物碱含量测定新方法。方法: 采用高效毛细管电泳法测定。结果: 苦参中生物碱能较好分离, 加样回收率苦参碱为97.47%, 氧化苦参碱为97.67%。结论: 该方法简便、准确、重现性好, 可作为苦参中生物碱的质量控制方法。

OBJECTIVE: To develop a simple and rapid method for the determination of alkaloids in Radix Sophorae Flavescentis (RSF). METHOD: HPCE buffer: 20 mmol.L-1 phosphates, pH 7.0; V = 20 kV, leff = 50 cm, L = 64.5 cm, i.d. = 50 microns, lambda = 201 nm, T = 25 degrees C, sample size = 10 kPa.s. RESULT: Matrine(I) and oxymatrine(II) in RSF were completely separated with recoveries of 97.47% and 97.67% respectively. CONCLUSION: Simple, accurate and reproducible, this method can b...