期刊论文

He, F.-Y.

中文

中国中药杂志

1001-5302

2008

33

6

642-648

10.3321/j.issn:1001-5302.2008.06.007

国家自然科学基金（30572378）

本校为其他机构

目的：创立HPLC测定中药成分溶度参数的理论与方法。方法：运用色谱理论及Hildebrand-Scatchard溶解度理论创立HPLC法测定化合物的溶度参数的数学表达式，用已知溶度参数的咖啡因验证该理论并对大黄类成分进行测定。HPLC条件，色谱柱为Alltech Apollo C_(18)柱（4. 6 mm×250 mm,5 μm）；流动相为乙腈-水，梯度洗脱；检测波长咖啡因270 nm，大黄类成分254 nm；柱温25 ℃；进样体积10 μL。结果：建立了保留时间与溶度参数关联的数学表达式，用此测得咖啡因的溶度参数为28. 31 J~(1/2)·cm~(-3/2)，与文献28. 84 J~(1/2)·cm~(-3/2)基本一致，用此法测得芦荟大黄素、大黄酸、大黄素、大黄素甲醚...

OBJECTIVE: To establish the theories and methods to determine solubility parameters of multiple components for the traditional Chinese material medica (TCMM) with HPLC. METHOD: The mathematical expresses to determin the solubility parameters were established according to chromatographic and Hildebrand-Scatchard theories, The HPLC experiments were carried out at 40 degrees C on an Alltech Apollo C18 column (4.6 mm x 250 mm, 5 microm) eluted with acetone and water in gradient mode. The flow rate was 1.0 mL min(-1), and the detection wavelength was 254 nm. The injection volume was 1 0 microL. RES...