期刊论文

Feng, X.

中文

中国中药杂志

1001-5302

2011

36

22

3119-3124

10.4268/cjcmm20112212

中医药行业科研专项（200807054）；

本校为第一且通讯机构

目的:建立13批板蓝根超微粉体水溶性成分和氨基酸柱前衍生化双重HPLC指纹图谱。方法:采用RP-HPLC梯度洗脱,选择Hypersil BDS C_(18)色谱柱(4.6 mm×250 mm,5μm);水溶性成分HPLC指纹图谱的流动相乙腈-0.1%磷酸水溶液,流速0.5 mL.min~(-1),柱温20℃,进样量10μL;氨基酸成分HPLC指纹图谱样品经异硫氰酸苯酯试剂柱前衍生化处理后进样,流动相0.1 mol.L~(-1)醋酸钠缓冲溶液(pH 6.5)-乙腈,流速1.0 mL.min~(-1),柱温30℃,进样量5μL;检测波长均为254nm。采用国家药典委员会"中药色谱指纹图谱相似度评价系统(2.0版)"处理分析,建立板蓝根指纹图谱,同时测定了4种主要成分含量,并进行SPSS系统聚类分析。结果...

OBJECTIVE: To establish the double HPLC fingerprints of water-soluble composition and amino acids precolumn derivative reagent of 13 batches of Isatidis Radix micropower. METHOD: The gradient elution was adopted with Hypersil BDS C18 column (4.6 mm x 250 mm, 5 microm). Water-soluble ingredients were detected with acetonitrile-0.1% phosphoric acid solution as mobile phase, flow rate 0.5 mL x min(-1), column temperature 20 degrees C, and the injection volume 10 microL. Amino acid ingredient were derived by PITC, and then were detected with mobile phase of 0.1 mol x L(-1) sodium acetate buffer so...