期刊论文

Developing spectrum-toxicity relationship with rough set theory for hepatotoxicity material basis of Polygonum multiflorum

Lin, L.-M.

中文

中国中药杂志

1001-5302

2019

44

3

509-517

10.19540/j.cnki.cjcmm.20181114.001

国家自然科学基金项目(81503041,61502538) 中医药行业科研专项(201507002) 湖南教育厅一般项目(17C1213) 湖南省重点实验室开放项目(20160207).

本校为第一且通讯机构

何首乌特异质肝毒性是行业关注的热点问题之一.该研究采用MTT法和流式细胞术筛选出何首乌特异质肝毒性最强的毒性部位为其醇提液大孔吸附树脂50%乙醇洗脱部位(简称何首乌50%乙醇部位);采用HPLC建立不同产地生/制何首乌50%乙醇部位指纹图谱,有14个共有峰;采用MTT法检测上述样品对人正常肝脏LO_2细胞的生长抑制率;通过粗糙集理论进行二者的“谱毒”相关性分析,预测出对特异质肝毒性有贡献的2个主要峰,其中贡献最大的为二苯乙烯苷;体外特异质肝毒性验证实验结果表明二苯乙烯苷是何首乌特异质肝毒性的主要物质基础.以上研究结果对揭示何首乌特异质肝毒性物质基础具有现实意义,同时也表明粗糙集理论可...

Idiosyncratic hepatotoxicity of Polygonum multiflorum has attracted a great attention in the world. The most toxic part of idiosyncratic hepatotoxicity was screened by MTT assay and flow cytometry, which was the 50% ethanol elute by macroporous adsorptive resins from alcohol-extraction of P. multiflorum. The fingerprints were collected by HPLC from 50% ethanol elute of crude and processed P. multiflorum from different habitats, then 14 common peaks were determined. Spectrum-toxicity relationship was analyzed by rough set theory(RST). Two main chemical components were predicted for idiosyncrati...